I am trying to do de novo genome sequencing for a Basidiomycetes fungal isolate using PacBio CLR, as we already failed HiFi Pacbio and still hoping to get long-read sequences. We used CTAB method together with Qiagen Genomic-tip for the HMW DNA extraction, and we saw bands > 25 kb when we run the agarose gel. After we shipped the sample to the sequencing company, they run Qubit and found the sample was degraded with the main fragment < 15 kb so it is not even worth to proceed library prep. I wonder if anyone also had the same problem with similar species. I can't figure out what caused it because we did the same extraction and shipped another isolate at the same time, that one did not have a problem and was successfully sequenced.