Dear All,
I am trying to grow M.bovis bcg for electroporation on 7H9 liquid medium supplement with tween80, ADC, sodium pyruvate and casitone or 7H10 solid agar supplement with ADC, sodium pyruvate and casitone. Both medium were autoclaved at 120oC 10min. However, I found that my culture keep on being contaminated. Do you guys know if there is any way to prevent contamination like adding bacterial inhibitors eg. malachite green?
Thank you.
Ellen
Ellen Chu Hi, you can add cycloheximide to your culture medium.
Dear Ellen Chu, Mycobacterium is usually resistant to it, although don't have an idea of electroporation. Even though can assure you it won't have any drastic effect on the cell structure. you can give it a try if didn't find any other better option.
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