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Questions related from Ellen Chu
Dear All, I am now working on a protein that is heterogeneous when it is in solutions. However, after I crosslink it with DSSO, it will form a homogenous ring when observing under TEM. Therefore,...
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Dear All, I would like to determine the stoichiometry and Kd of few types of protein complexes which will consume quite a lot of capillaries and I think my boss may kill wanna kill me for...
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Hi everyone, I have 2 proteins that known to have interaction. However, they cannot form stable complex when I overexpress and purify the from E.coli. Most of the time I can only observe...
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Dear All, I'm now trying to amplify a gene with 3690bp from the genomic DNA. I've design 3 sets of primers. First set is flanking the whole gene. Second set is flanking from 1 to 2613bp. Final...
08 February 2019 1,288 1 View
Hi everyone, I am now following a silver staining protocol which will add 20ul formaldehyde together with 0.125g silver nitrate in 50ml water. However, according to my knowledge formaldehyde...
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