Dear all,
I have performed an ELISA assay for which there are many data points at the lower limit of detection. When presented on an xy scatter plot these points at the LLOD on the x-axis cluster such that they are indistinct / merge which, is not ideal. I have tried making the data points smaller but they would have to be extremely small to be individually distinct making the plot very difficult to read.
Is there another legitimate way to present them within this plot?
Could I present these plots within a clearly defined and marked narrow vertical rectangular area rather than on a line?
Your ideas appreciated,
James
The best option I can see is using a discontinuous axis where at the lower values there is finer spacing. Similar to Example 3 on this page https://www.graphpad.com/guides/prism/latest/user-guide/discontinuous_and_hidden_axes.htm
another alternative is to plot all values and then use a second plot (as a pop out) that is fitted for the lower values. although a discontinuous axis is legitimate, many readers miss the break in the axis and therefore interpret the plot incorrectly.
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