How to prepare water saturated ethyl acetate?
Simple easy important prep
In general 3.3 mL of water will dissolve in 90 mL of anhydrous ethyl acetate.. so mix water and ethyl acetate accordingly to ur needs and remove the ethyl acetate after well mixin!! :)
I have had prepared serial dilutions of 3MCPD standard and internal standard (3MCPD-ds) for calibrating my GC-MS (Agilent ) according to EU 14573:2004. But after injection I got nothing. Non of...
03 April 2013 214 3 View
I have prepared 3mcpd in 20% NaCl solution standard and 3mcpd-d5 in water as internal standard. After injection on my GC-MS 5975, the ion of 3MCPD appeared with good response but with the...
02 March 2013 1,688 11 View
Yesterday I came to my instrument ABSciex Triple Quad 5500 coupled to Agilent HPLC 1290 to initialize the profile, but it kept giving me an error message that said: "Failure to initialize binary...
01 February 2013 6,376 11 View
There is a plan to introduce Dioxin analysis in our Lab. I need your recommendation for Which instrument we should go for.
31 December 2012 7,496 6 View
SOP ( Standard Operating Procedure )
10 November 2012 8,322 4 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...
02 March 2021 7,626 1 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
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I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...
01 March 2021 1,587 6 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...
24 February 2021 166 6 View
Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...
24 February 2021 2,602 1 View