How to prepare water saturated ethyl acetate?

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Missing peak and ions of 3MCPD

I have had prepared serial dilutions of 3MCPD standard and internal standard (3MCPD-ds) for calibrating my GC-MS (Agilent ) according to EU 14573:2004. But after injection I got nothing. Non of...

03 April 2013 273 3 View

3MCPD-d5 ions missing.

I have prepared 3mcpd in 20% NaCl solution standard and 3mcpd-d5 in water as internal standard. After injection on my GC-MS 5975, the ion of 3MCPD appeared with good response but with the...

02 March 2013 1,769 11 View

HPLC failure.

Yesterday I came to my instrument ABSciex Triple Quad 5500 coupled to Agilent HPLC 1290 to initialize the profile, but it kept giving me an error message that said: "Failure to initialize binary...

01 February 2013 6,591 11 View

Dioxin analysis

There is a plan to introduce Dioxin analysis in our Lab. I need your recommendation for Which instrument we should go for.

31 December 2012 7,558 6 View

I am searching for a SOP concerning steroids analysis in meat products using LCMSMS, Can anyone help?

SOP ( Standard Operating Procedure )

10 November 2012 8,397 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View