How to prepare the iodine dye used for pigmentation the Eimeria parasite?

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Khadeeja Abees Hmood Al-Khalidy

see this link

https://www.cartercenter.org/.../pdfs/.../ln_parasitology_final.pdf

Sadiya aziz Anah

Thank you, Khadeeja Alkhalidi

Fatima Ibrahim Mohammad Allebawi

dear Sadiya:

Iodine Wet mount

a. Place a drop of Lugol's iodine solution on a slide.

b. Pick up a small amount of fecal material on an applicator stick using the same criteria in the saline procedure for selection of the proper areas.

c. Emulsify in the iodine solution and cover with a coverslip.

d. Examine on low and high power as described in the previous procedure.

NOTES: The film may be made directly with iodine or the iodine may be added to a saline mount by adding a drop to the edge of the coverslip so that it gradually diffuses into the saline mount.