serum consist from water , salt and antibodies but can use in pcr technique
You can use serum in a PCR assay. Usually you must first do RNA or DNA extraction using a phenol-chloroform method (guanidinium thiocyanate-phenol-chloroform method for RNA or proteinase K-phenol-chloroform method for DNA) because it controls better for PCR inhibitors. However good PCR performance has been described using serum directly. If you try this, I would advise to try to use the minimum amount of serum to reduce PCR inhibition by serum proteins.
Nevertheless, I think you should probably do a pilot experiment in order to check for adequate reaction efficiencies and reliability from controls.
There are several available protocols online.
best luck
yes you can use serum in direct PCR assay and this link explain application to Viral Genome Detection as example to direct PCR from Serum
link.springer.com/protocol/10.1385%2F1-59259-384-4%3A161
Best luck
yes , but usually at least 3 h are needed for this step. Detection of RNA is more difficult and complex than that of DNA, mainly because of the contaminating RNase and the need to conduct an additional reverse transcription (RT) step. Additionally, another problem is the high cost of the reagents for RNA extraction.
If you want to know, whether serum can be used directly without sample preparation in PCR, the textbook answer is: No. However, some work has been performed to overcome these limitations and today some test kits are available commercially from different companies.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871721/
http://link.springer.com/protocol/10.1385%2F1-59259-384-4%3A161
https://www.thermofisher.com/order/catalog/product/F547S
http://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(14)64030-1/abstract
http://www.creative-biogene.com/Blood-Direct-PCR-Kit-DPZ-02-1234410-87.html
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