I have to isolate actinomycetes from soil, for which soil extract agar is required..It would helpful if a protocol is available to prepare soil extract. Not for soil extract agar
The soil extract can be prepared by mixing 5g of soil in 1L H2O mix well, filter and then autoclave.
You don't have to use soil extract to isolate Actinomycetes you can use any other medium that contained two different antibiotics for inhibition of fungi such as nystatin and streptomycin for inhibition of bacteria.