Should one always use desiccated ammonium sulphate for salt precipitation?

Some of the protocols have mentioned, heat the ammonium sulphate at 120 degree C overnight and grind it to a fine powder before use. But most of the protocols do not mention such step. Is it...

07 August 2018 6,861 0 View

How to find the score of the alignment ?

Assuming an affine gap scoring scheme of an identity matrix for substitution, a gap initiation penalty of 1 and a gap extension penalty of 0.1, the score of the alignment would be ?...

04 May 2018 6,597 0 View

How to prepare soil extract for using it in a medium ?

I have to isolate actinomycetes from soil, for which soil extract agar is required..It would helpful if a protocol is available to prepare soil extract. Not for soil extract agar

07 August 2016 6,723 3 View

I want to whether SCIENCE journal has publication charges or not ?

No info regarding the fees have been mentioned in the science page.

06 July 2016 8,771 1 View

Can Antifungal and Antibacterial agents / Antibiotics be added in the media before sterilization or after the media is sterilized ?

06 July 2016 1,916 2 View

What is the exact difference between plant meristem and plant stem cells ?

02 March 2016 4,750 3 View

Can anyone suggest how to prepare CM Cellulose for gel filtration chromatography ?

01 January 1970 7,747 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View