Moni Philip Jacob K

8 Questions 8 Answers 0 Followers

Questions related from Moni Philip Jacob K

Should one always use desiccated ammonium sulphate for salt precipitation?

Some of the protocols have mentioned, heat the ammonium sulphate at 120 degree C overnight and grind it to a fine powder before use. But most of the protocols do not mention such step. Is it...

08 August 2018 6,928 0 View

How to find the score of the alignment ?

Assuming an affine gap scoring scheme of an identity matrix for substitution, a gap initiation penalty of 1 and a gap extension penalty of 0.1, the score of the alignment would be ?...

05 May 2018 6,645 0 View

How to prepare soil extract for using it in a medium ?

I have to isolate actinomycetes from soil, for which soil extract agar is required..It would helpful if a protocol is available to prepare soil extract. Not for soil extract agar

08 August 2016 6,813 3 View

Can Antifungal and Antibacterial agents / Antibiotics be added in the media before sterilization or after the media is sterilized ?

I have to selectively isolate Endophytic Actinomycetes from a plant . In order to prevent the growth of fungi and bacteria, in papers , Antibiotics are added. Should that be added Before / After...

07 July 2016 2,016 2 View

I want to whether SCIENCE journal has publication charges or not ?

No info regarding the fees have been mentioned in the science page.

07 July 2016 8,817 1 View

In papers for isolating endophytic actinomycetes antibiotics are used .. I need to know the exact concentration to be used ?

It was mentioned that 60 microgram per millilitre is used . wont the concentration be a bit high ?

07 July 2016 930 6 View

What is the exact difference between plant meristem and plant stem cells ?

Are they similar or different ?

03 March 2016 4,812 3 View

Can anyone suggest how to prepare CM Cellulose for gel filtration chromatography ?

I am working to purify enzyme using chromatography. Our lab does not have sephadex or sepharose. some papers have used CM cellulose for purification of the enzyme of our interest. But most of the...

01 January 1970 7,804 5 View