Hi Ahmed, I think Przemyslaw gives some pretty good suggestions. The key to getting a good zeta potential measurement is to (as much as possible) achieve a single particle suspension. If your particles stick to one another, this will lower the zeta potential as there will be less surface charges to measure. Ultrasound sonication can be useful for this but above all I would suggest you find the best suited buffer for your particle of interest. HBS and PBS have worked great in my experiments. Also, make sure that your concentration is high enough to detect a signal but low enough to prevent aggregation.
Come up with an estimation of how many surface charges you should have before doing the experiment. If you estimate that you should have a surface charge and continue to get values -10>x
What's the adequate preparation for measuring zeta potential : My sample is MgO?
Hi. I would ask about food sample preparation for Zeta Potential and Partical size!
How to dilute food emulsion for Zeta potential test?
Does all food emulsion need Ultrasonication ?
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