In my cell experiments, I used a 10% concentration of probiotic supernatant (diluted with 90% PBS). I would like to know if the same concentration can be used in mouse experiments.
However, I am concerned that 10% may not be effective enough. Should I increase the concentration for better results? Unfortunately, I couldn’t find any relevant literature for reference.
I would really appreciate your advice on this. Thank you!
Hello, Lai
As you using supernatant you need to check the concentration of the active substances like Bacitracin then you can decided which level you have to use
Hi Lai,
probiotics bacteria produce different substances that made probiotics active agent so, you need to find which one of them you going to focus on or if you just want to check if this produce works or not you can chose many levels like 5, 10 and 15% ( this just suggestion im not sure as didnt work on eye). if there is no papers published on this works you can try it first in pilot study. take different levels put on their eye then see what happen if no inflammation you can go ahead
best wishes
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