Oligo dT magnetic beads are usually used to isolate poly A mRNA from total RNA. But commercial Oligo dT magnetic beads had fixed sequence, I could not change it to my usage.
Anyone knows how to self prepare oligo dT magnetic beads from special oligo dT and magnetic beads. How to choice empty magnetic beads? How to design special oligo dT (I had known sequence)? How to conjuncate oligo with beads?
Use an expoxy or caboxylated coated magnetic bead and a amine modified oligo with your sequence. Another approach would be a streptavidin coated bead and a biotinylated oligonucleotide.
Streptavidin Dynabead (www.thermofisher.com/dynabeads) + order a biotinylated oligonucleotide of any sequence (eg from IDT). binding is pretty much instant
yes, with the biotin/strep solution one has full control over where on the oligo the biotin is inserted and hence you will get the same orientation for all the immobilized oligos. The interaction between streptavidin and biotin is the strongest non-covalent binding known with association constant of 10e15 M-1.
Ketil Winther Pedersen I have just read the discussion, is there any written protocol for it?
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