Stones King

3 Questions 5 Answers 0 Followers

Questions related from Stones King

Method or protocol for deletion of gene in Bacillus subtilis?

One gene in Bacillus subtilis should be deleted in my lab. Any confidity method or protocol or kit can be used? Is it same as operation for E. coli? What should I pay more attention?

30 June 2016 4,637 18 View

Can water molecule be included as ligand in the activity pocket when making molecular dynamics simulation?

When MD simulation, all water molecules are excluded inside protein (except binding with cofactors). Now my protein has no cofactors, but I want to know how the protein behave once water inside...

10 February 2016 9,646 3 View

How to make molecular dynamics simulation when a non-standard molecule covalently bind with protein?

I want to make MD simulation of a system which ligand (non-standard) covalently bind with protein. Any protocol can be provided? I used to make it as non-covalent binding ligand, but after MD...

30 January 2016 4,365 5 View