Hi,
I want to check the level of Tryptophan oxidation in my protein. For this experiment I want to use fenton's reagent with 0.1mM Fe2+, 0.043mM and 0.05M H2O2. please help in preparing the reagent.
Thanks in advance.
For 0.1 mmol Fe2+ = 0.00585 gm
0.043 mmol H2O2= 0.0014 gm
0.05 mmol H2O2= 0.0017 gm
You can also prepare by this method also
The Fe2+ catalyst for the Fenton's Reagent was prepared by dissolving 69.50 g of analytical-grade ferrous sulfate (FeSO4.7H2O) in 500 mL of sterilized deionized water (DI) containing 5 mL of concentrated H2SO4 .
For further information you can check following link
http://shodhganga.inflibnet.ac.in/bitstream/10603/15518/11/11_appendix.pdf
Dear Manjula,
The formulation of you question clearly indicates that you do not understand the complexity of your system. Tryptophan + Fenton depends on pH, Fe(II)/Fe(III) form complexes with tryptophan, etc. The problem is not in preparing solutions, but in data interpretation.
Dear Shivani,
You calculations are based on 100% H2O2, which is not commercially avalaible. The H2O2 concentration in commercially available solutions should be determined experimentally.
the following articles and link may be helpful for you
Fenton, H.J.H. “Oxidation of tartaric acid in presence of iron”, J. Chem. Soc., Trans., 65pp. 899-911, 1894.
Fenton's reagent revisited
Cheves Walling
Acc. Chem. Res., 1975, 8 (4), pp 125–131
https://www.youtube.com/watch?v=iKHuaCR6aF0
Fenton’s reagent is the name given to the reaction of a solution of hydrogen peroxide and a ferrous iron catalyst. Companies often use Fenton’s reagent to reduce the levels of organic contaminants through the chemical oxidation that occurs as the solution produces hydroxyl radicals. Making your own Fenton’s reagent can allow you to experiment with removing chlorinated organics and similar organic contaminates in the safety of your own laboratory.
Add buffers to your solution to lower the pH level between 3 and 5. Test the pH several times to ensure that the solution’s acidity has equalized.
Pour a solution of ferrous sulfate (FeSO4) into the solution you wish to oxidize with the Fenton’s reagent.
Slowly add hydrogen peroxide (H2O2) to the solution. Check the pH and adjust the rate at which you add the hydrogen peroxide in order to keep the solution’s pH between 3 and 6.
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