How to prepare Ascorbate buffer pH 6.8?

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How to standardize growth curve of nitrogen-fixing cynobacteria?

I am working on nitrogen-fixing fresh and marine water cyanobacteria. I want to estimate proline. Whether I should standardize the OD to ensure the exponential phase or the time limit for growth...

09 October 2016 5,800 3 View

How can I include soil Parameters in prediction using MaxEnt?

I am using MaxEnt software for prediction of 'presence' of the plants using ground data (GPS readings). Although, MaxEnt uses BioClim layers, it doesn't consider any soil parameter, Are there any...

31 December 2014 3,154 2 View

How to calculate H2O2 concentration in plant extract?

Plant material extract was prepared in PCA. Then, it is treated with Potassium thiocynate and ferrous ammonium sulphate. OD at 480 nm. How to calculate the amount of H2O2 in the plant extract?...

05 June 2014 7,766 3 View

How many components can I retrieve in principal component analysis?

I am doing PCA of a data of 9 variables. How many components can I retrieve from these variables? Do I have a choice to have the components 'of-my-choice'? I am using SPSS software for the same.

11 December 2013 1,490 15 View

How can I get the sharp bands in PAGE?

In PAGE, I prepared 4% stacking gel and 12% resolving gel. I am not getting sharp bands and its 'trailing'. What should I change to get sharp bands? Can you please help me?

10 November 2013 5,094 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View