How to prepare Ascorbate buffer pH 6.8?

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How to standardize growth curve of nitrogen-fixing cynobacteria?

I am working on nitrogen-fixing fresh and marine water cyanobacteria. I want to estimate proline. Whether I should standardize the OD to ensure the exponential phase or the time limit for growth...

09 October 2016 5,889 3 View

How can I include soil Parameters in prediction using MaxEnt?

I am using MaxEnt software for prediction of 'presence' of the plants using ground data (GPS readings). Although, MaxEnt uses BioClim layers, it doesn't consider any soil parameter, Are there any...

31 December 2014 3,226 2 View

How to calculate H2O2 concentration in plant extract?

Plant material extract was prepared in PCA. Then, it is treated with Potassium thiocynate and ferrous ammonium sulphate. OD at 480 nm. How to calculate the amount of H2O2 in the plant extract?...

05 June 2014 7,890 3 View

How many components can I retrieve in principal component analysis?

I am doing PCA of a data of 9 variables. How many components can I retrieve from these variables? Do I have a choice to have the components 'of-my-choice'? I am using SPSS software for the same.

11 December 2013 1,559 15 View

How can I get the sharp bands in PAGE?

In PAGE, I prepared 4% stacking gel and 12% resolving gel. I am not getting sharp bands and its 'trailing'. What should I change to get sharp bands? Can you please help me?

10 November 2013 5,209 7 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View