I would like to know how can I prepare some antibiotics disks for an antifungal sensitivity assay? I would like to investigate the antifungal activity of some formulations that I have developed for vaginitis using the Diffusion Susceptibility Testing of Yeasts.
Hi Samyr,
Prepare the desired discs using solutions of your products with concentrations related to the desired quantity in each disc. Take in consideration the volume to be put in each disc is small (20 to 50 microliters) to facilitate the manipulation.
example: if you want to put 100 microgrogrammes in the disc. Prepare a solution with concentration 2 mg/ml. Then you put 50 microliters in the disc for 100 microgrammes, 25 microliter for 50 microgrammes.
Dear Samyr,
attached is a Manual on Antimicrobial Susceptibility Testing.
Good luck
Hi Samyr,
Prepare the desired discs using solutions of your products with concentrations related to the desired quantity in each disc. Take in consideration the volume to be put in each disc is small (20 to 50 microliters) to facilitate the manipulation.
example: if you want to put 100 microgrogrammes in the disc. Prepare a solution with concentration 2 mg/ml. Then you put 50 microliters in the disc for 100 microgrammes, 25 microliter for 50 microgrammes.
You can perform in vitro drug sensitivity test of yeast isolates either by preparing disc for any compound as informed by Noureddine or by purchasing antifungal drug sensitivity disc from Hi-Media,India.If you have shortage of fund,I advise you to prepare disc from filter paper,which we frequently use in our Public Health and Microbiology Laboratory.You can prepare disc from Mercurochrome,Gential violet ,which also work against some fungi. You can try to do antifungal drug sensitivity test on our two mediaum: one Pal sunflower seed medium and other is APRM agar.I have uploaded over 350 papers on Research Gate and Academia website.There are many publications related with fungal infections.You may download as per your interest.
With best wishes and good luck,
Prof.Dr.Mahendra Pal, Founder Director of Narayan Consultancy on Veterinary Public Health and Microbiology, Anand, India
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