I have to prepare a culture media for S. cerevisiae (YNB without amino acids, supplemented by a drop out mini 4 different aa, and then have to add some aa).
I cannot really find any standardised protocols for the preparation of amino acids stock solutions.
- Which stock and final concentration? Is it depending on the aa? If so, I need: trp, ura, leu, his
- How to dilute them?
- How to store stock solutions? (Volume of aliquots for convenient work, which temperature...)
Thank you in advance for your precious help!
Thank you! For the dilution, I guess they're not all the same, I can't dilute them all in water?
A colleague told me to store them at 4°C, is it ok?
Dear Noemi,
Usually I prepare stocks (2 mg/ml his, 6 mg/ml leu, 4 mg/ml trp, 2 mg/ml ura) just by disolving amino acids in water. Stock solution should be sterilized by autoclaving and can be stored at room temperature. To grow strains in W303 or BY background I add 1 ml of AA per 100 ml of medium.
Best regards
Irek
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