6 Questions 23 Answers 0 Followers
Questions related from Noémie Jelly
Dear all, I am facing Subit issues, for mosquito genomic DNA measurements (for gDNA library preparation for NGS). The same sample was measured by 3 different people (in december 2015, august 2016,...
27 February 2017 2,954 5 View
We are facing a problem in different groups in our institute: when we grow E. coli (DH5a) overnight in liquid LB, we have: - no growth at all - sometimes, some tubes show cell growth, some...
21 February 2017 2,542 12 View
I have genomic DNA eluted in TE buffer, and am planning to sequence them. Unfortunately, EDTA should be avoided for Nextera library preparation. I am thus planning to purify my gDNA again, using...
20 March 2015 5,184 22 View
Dear all, I would like to sequence genomic DNA with the Nextera DNA sample preparation kit. For those who used it, you know for sure that it's very expensive! We were thus wondering whether it was...
06 March 2015 4,028 2 View
I have to prepare a culture media for S. cerevisiae (YNB without amino acids, supplemented by a drop out mini 4 different aa, and then have to add some aa). I cannot really find any standardised...
12 March 2014 4,854 3 View
I would like to write a review and add figures including pictures of different publications. I was wondering how scientists usually react if one asks them whether they can send one of these...
10 August 2013 3,391 3 View
