Any one familiar with SOFC/SOEC simulations using Ansys Fluent ?

23 August 2020 8,231 3 View

Adjust annealing temperature or additives to enhance specificity of PCR?

Dear All, I have a very basic question about the PCR/RT-PCR/qPCR. The PCR primers and TaqMan probes are designed such that the primers/probe connot bind to the mismatched site but only...

15 June 2020 4,926 3 View

Crystallization of PLLA?

07 June 2020 2,395 6 View

How to define grain boundary hardness?

08 March 2020 3,388 11 View

Will the TaqMan probe length affect the fidelity of the qPCR assay?

Dear all, I am learning to design the qPCR assays recently. But I just don't know why my negative controls always give positive signal( I have changed my design twice). I checked the formation of...

07 October 2019 4,968 3 View

Effect of mRNA secondary structure on reverse transcription?

Dear Sir/ Madam, I am designing a primer for microRNA reverse transcription. But some microRNAs like hsp-mir-21-5p has a strong secondary structure. It forms a stable hairpin. Can anyone please...

08 September 2019 8,283 3 View

How to deal with the nonlinearity in electrical tomography?

The electrical tomography can be utilized to calculate the solid concentration or gas holdup in aqueous based multiphase flows. However, there usually exists a strong nonlinear relationship...

16 May 2019 197 1 View

How many base-pairs can be added to the pSin vector?

Recently I decide to add a 5.5 kb fragment to pSin vector will it affects the efficiency of virus packaging?

20 March 2019 9,786 2 View

How can I deal with the non-specific binding in XIST-RNA-FISH?

I'm trying to detect the XIST-RNA expression in Breast cancer cells using the Biotin and Digoxin probes. However, there are always non-specific bindings in my results. I have added RNase-inhibitor...

18 March 2019 3,761 1 View

Any UV light absorbing material at 193-248 nm?

Would like to coat the alloy surface with non-organic material to absorb the UV light at 193-248 nm. Please recommend some UV absorbing material. Thanks~

16 November 2018 1,466 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Which is the most ecofriendly and effective activated agent for the preparation of activated carbon from sewage sludge?

I am going to prepare activated carbon from the waste activated sludge coming out of WWTP. Can you please help me in understanding, which chemical/physical activating agent will be most...

07 February 2021 9,507 5 View

What is the reason of suddenly increasing the concentration while evaluating the photocatalytic performance by RhB dye degradation?

I am doing a photocatalytic performance evaluation by RhB dye degradation. I am facing a problem which is: After achieving the absorption -desorption equilibrium, I checked the concentration and...

04 December 2020 3,922 20 View

What is the influence of reduced graphene oxide (rGO) in a p-n ternary photocatalyst?

04 December 2020 9,019 2 View

Why in BET surface area becomes zero but when detag ads des, it shows good value around 150-200? For single crystals is it a good data to publish?

For single crystals can I give BET data in the publication if it is coming around 200. And why it shows zero value surface area but after detag ads or des it shows value, and which one I should...

02 December 2020 7,173 1 View

How to remove TiO2 nanoparticles from dye solution, in order to acquire an absorbance graph for the dye alone ?

I'm working on the degradation of various pollutants with bare and composite TiO2. Since the size of TiO2 is too small, the particles won't be settling down as we take the dye solution at...

01 December 2020 2,139 8 View

How Important Is the Rate of Heating During the Synthesis of Carbon Nitride?

09 November 2020 6,096 2 View

What is the equation used in the calculation of surface area from BET plot?

I want to know the equation that I should use when I calculated the surface area using a plot like this one in the attachment. What are the parameters required for the calculation?

15 October 2020 8,364 6 View

Is K-silicate gel a porous material?

I coated a stone surface with K-siilcate. Apparently, it does noe seal the surface since the water penetrae the stone easily and the absorption coefficient does not change too much. However, the...

05 October 2020 3,435 5 View

Can we use Aluminum sheet as a substrate for thin film and have surface area calculated from BET?

I am to calculate surface area of a thin-film using BET and use the same thin-film for electrochemical analysis. I have chosen aluminum sheets, will there be any issues with BET??

27 September 2020 8,456 4 View