I am PhD candidate, I want to induce colon cancer through intraperitoneal injection of 40 mg/kg of dimethylhydrazine in male Wistar rats . However, I would appreciate it if you could provide further clarification on the steps of 40mg/kg dimethylhydrazine preparation from stock bottles of the dimethylhydrazine reagent because it comes in liquid form (not in solid form). Understanding this in more detail would greatly assist me in accurately implementing the method in my PhD work.

If you have any supplementary materials, protocols, I would be extremely grateful.

Thank you for your time and for contributing such valuable work to the field.

Best regards,

Rabiu Musa Isah PhD Candidate, Department of Human Physiology Faculty of Basic Medical Sciences, Bayero University Kano, Nigeria

phone Number (+2348032952725)