For example the drug is highly hydrophillic and the lipid is hydrophobic in nature. When I add lysing solvents like ACN, methanol, tritonX100, the lipids are getting dissolved in the solvent. And since I need to estimate the drug content by LCMs method and the lipids should not be present in the sample solutions, I kindly request to advice the suitable method to do the separation.
Lipids are difficult to precipitate. Solid Phase Extraction (SPE) could work or do a classical solvent extraction with repeated partitioning between two immiscible solvents. Detergents like TX100 are a nightmare to get rid of. If possible, avoid them completely. There are some which may be separated at certain temperatures; if I remember correctly, Triton X 114 is one of them.
For all extractions, adding a tracer and spiking experiments might be essential to assess recovery.
Try a water immiscible solvent to dissolve the lipids, add water if necessary, separate the aqueous layer and estimate.
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs