Dear All,
I have to paraffin embed shavings of cartilage ...super small less than 1X1 mm in size for H&E staining. I will have a scoop of these shavings that will be glued together during culture using Evicel. After 4 weeks in culture I'll have to pick up the blob of glue with the cartilage and proceed for histology. Any pointers as to: duration of fixing in 4%PF - dehydration - embedding and sectioning? I will have two groups with and without glue. The scoop of cartilage without glue will be a challenge as the tiny pieces will be all over -floating around in media.Thanks-Anu
In histology core, they place smaller tissue in smaller sieved bags like tea bag pouch. They than process the the pouch thru all solutions so that tissue remain in pouch. There should not be worry. Talk to your pathology core lab, they can help you. I have done these with cartilages I made in vitro from stem cells which were 1 to 2 mm in diameter. Good luck
Thank you. Any suggestions as far as the orientation of the tissue is concerned and the sectioning?
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