Hello,
I need to stain a 8mm ( wide) and 10 mm (deep) piece of human femoral chondyle...no cartilage just bone.. This is the left over piece from the cadaver donor joint. If someone can share a tested protocol for the same.
Thanks
Dear All, I have to paraffin embed shavings of cartilage ...super small less than 1X1 mm in size for H&E staining. I will have a scoop of these shavings that will be glued together during...
03 October 2019 5,394 2 View
Dear All, IHC staining done -Heat retrieval-Blocking -Primary Ab overnight-Secondary ab -ABC kit-DAB- dehydration(Xylene 5 mins ) and mount. I loose lots of sectionns During the xylene...
01 May 2019 4,633 20 View
Dear all, I have to section slides and keep them ready for IHC from 100+ blocks of paraffin embedded Goat and Human articular cartilage. How early can I section and store my slides before IHC...
14 April 2019 7,976 17 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...
24 February 2021 4,618 4 View