Hello,
I need to stain a 8mm ( wide) and 10 mm (deep) piece of human femoral chondyle...no cartilage just bone.. This is the left over piece from the cadaver donor joint. If someone can share a tested protocol for the same.
Thanks
Dear All, I have to paraffin embed shavings of cartilage ...super small less than 1X1 mm in size for H&E staining. I will have a scoop of these shavings that will be glued together during...
03 October 2019 5,466 2 View
Dear All, IHC staining done -Heat retrieval-Blocking -Primary Ab overnight-Secondary ab -ABC kit-DAB- dehydration(Xylene 5 mins ) and mount. I loose lots of sectionns During the xylene...
01 May 2019 4,688 20 View
Dear all, I have to section slides and keep them ready for IHC from 100+ blocks of paraffin embedded Goat and Human articular cartilage. How early can I section and store my slides before IHC...
14 April 2019 8,069 17 View
IN recent times.i.e. in the last ten years, there have been numerous case reports on Head and Neck Squamous Cell Carcinoma which may have HPV as one of the contributing/causative agents. I have...
21 July 2015 3,860 5 View
I want to do a pilot study on the dental conditions/ oral conditions of parsees over the age of 60 years. Seeking constructive suggestions from others who have knowledge of this kind of study/project.
02 September 2013 5,480 8 View
All dentists look at teeth, check for cavities, etc.and totally ignore or neglect the mucous membrane.
01 January 2013 9,504 30 View
Most of these are squamous cell carcinomas, and very well-differentiated, with lymph nodes of neck also involved. There is always a boy-friend or girl-friend in the picture.
25 December 2012 7,825 32 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View