Can anyone recommend an RNA extraction protocol from Cacao tissue (leaves, meristem, branch)?

Due Covid-19 I personally cannot visit a rural site for the collection of samples. But have found some great people who are happy to do the extractions on my behalf. I need to provide them with an...

19 November 2020 4,049 3 View

How can I design allele specific primers when I have twp SNPs only few base pairs apart?

Hi there, I am trying to design allele specific primers for KASP assays to validate the SNPs from GWAS results. However, few of my SNP sequences have two or more SNPs only few base pairs apart and...

13 August 2019 9,759 4 View

How can I prevent the degradation of the DNA?

I have extracted DNA from cacao leaf tissue (freeze dried). I used CTAB buffer (5%) along with 1% B-mercaptoethanol and 3% PVP. I did the DNA extraction overseas and transported it as a pellet to...

16 January 2019 2,055 12 View

Why am not seeing any DNA bands from my samples?

I have extracted DNA from Cacao leaf tissue using CTAB, chloroform isoamyl alcohol method. When I quantified my samples on Nandodrop they show a conc. of 300-800 (ng/ul) in 50 ul of TE buffer. The...

15 January 2019 2,144 14 View

Is it okay If I donot remove RNAse from my DNA samples after treating it for an hour at 37 C?

I am trying to remove RNA contamination from my DNA samples. I gave RNAse treatment for DNA resuspended in 50 ul of TE buffer. After 1 hour of treatment I reprecipitated the DNA using 1/10 V of...

14 January 2019 5,817 2 View

Does keeping tissue sample (patient biopsies) in Trizol for a long time (>6 month) degrades DNA ? or Leads to sheared DNA?

I am trying to isolate RNA and DNA from the tissue biopsy stored in Trizol at -80 degree for more than 6 months . I isolated RNA using the standard Trizol method and obtained good quality and...

22 November 2018 2,446 2 View

Why are my gel pieces not getting destained for in-gel digestion?

My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.

07 May 2018 5,419 1 View

How to properly store the sds gel pieces for in gel digestion?

I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?

15 January 2018 1,120 1 View

Is proteomic data analysis in R (using RforProteomics) better than any other proteomic software?

My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?

04 January 2018 2,591 6 View

I want reference of first paper of NiO/ZnO nanocomposites?

i want to know from were work on ZnO/NiO nanocomposite had started.

26 September 2017 3,538 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Best excel chart to compare algorithms' running time?

Hi I want to compare the running time of robotic path planning algorithms. The grid size is 8X8. I have 100 scenarios of start and goal positions.. Each scenario is run in three obstacles ratio...

22 February 2021 621 1 View

How to open an old ".MTS" file containing spectroscopic data?

Hi supreme hive-mind of the internet, we have some old *.MTS files that are supposed to contain spectroscopic data, probably some coordinates and a long list of single line spectra. Basically,...

16 February 2021 9,385 2 View

Can anyone name this graph type?

I've attached a recreation of this graph type that I need to make for study reports. The clients have sent them to us in the past, but I am trying to make the reporting process more streamlined,...

15 February 2021 9,795 3 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

How to check if the PMF profile obtained from ABF simulation is correct?

I am doing ABF simulations to calculate ligand binding enegy. The PMF profile that I get using single long ABF simulation is attached as pmf_total.png. When I do this ABF calculation with 10...

10 February 2021 4,170 2 View

What is the Greedy windowed algorithm description?

Hi Dear Ph. D., I have carefully read your paper on anomaly detection in smart meters. Unfortunately, the research does not decribe the greedy windowed algorithm, neither its complexity, nor the...

10 February 2021 2,462 3 View