Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Why many biochar producers find it difficult to sustain in different parts of the world?

Biochar (solid product with more than 60% carbon as per IBI) has been extensively studied for its production, characterization, and applications. In spite of offering numerous benefits in diverse...

23 April 2020 9,534 1 View

Cloning a 4.3kb insert into 6kb vector?

I really need tips for cloning 5kb inserts into 6kb vector. I am trying the cut n paste method using REs. Which ligation ratio should I use? What precautions should I consider? I would be really...

24 November 2019 9,429 2 View

Hii everyone, my spss paired t test value is coming different when i calcualte manually. i have checked many times. which is correct

my sample size is 43. when i am applying in spss its coming 13.5 and manually 0.88, what should i do

31 May 2017 2,409 5 View

How to detect a conserved region in a particular genus for which a genus specific primer can be designed?

05 May 2017 8,682 2 View

Which metals ions form complex with sodium potassium tartrate?

Can +3 cations form tartrate complexes?

25 October 2015 3,094 3 View

Are there any model specification tests with panel data?

Hi I am working on count panel data. Is there any test i can use to test model specification. Linktest and ovtest are not applicable.

23 May 2015 9,295 2 View

Where do I start screening for RNA-protein interactions?

15 February 2015 1,980 4 View

How to calcuate the dose of virus needed to infect 25cc flask at 1000 TCID50?

05 November 2014 1,607 2 View

Can somebody please suggest some tools to perform Gene ontology t0 the third level using UniProt IDs of the genes ?

I have screened a cDNA library of swine. I want to perform gene ontology on the obtained gene sequences so that a pie diagram representinbg the proportion of genes participating in various...

14 October 2014 4,022 10 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View