There are planty ways to do that. if you already have done biofilms in 96 well plates, you can do in the same plates, grown the biofilm , remove plantonic cells, and then collet biofilm by pipeting with PBS and perform CFU count in agar. 

There is a special Deviace similar to 96 well plates, where you can calculate MBEC (Minimal biofilm erradication concentration) Here is the reference.

"The MBEC Assay System: multiple equivalent biofilms for antibiotic and biocide susceptibility testing.

Ceri H1, Olson M, Morck D, Storey D, Read R, Buret A, Olson B."

Also i have seen some report with microfermentator, but i dont now much about it. 

Alternative technique by fluorescent study by taking some agents/quenching chemicals

Dear Dinesh, you check biofilm formation on material surfaces by simple staining with crystal violet then visualize optic microscope or stereo zoom microscope. If it is available, you can use SEM and AFM techniques for detailed analysis

If you want to quantify biofilm cells on material surfaces, first you can remove unattached cells then detach biofilm formers by cell scapers and sonication. It depends on your material.

Best regards. 

but how to grow pathogens or develop biofilm ? on which material or surface I should grow it ?  I want to check my nanomaterial antibiofilm activitity.

I do not know a standardized method but you can wash the surface with dist water and dry at 40-50C  up to a constant value and weigh the sample and compare with the control. 

Hi Dinesh,

One of the easiest method is crystal violet staining. You can use polystyrene microtiter plate. For your reference, please check the articles the links of which are mentioned below. I hope that would be helpful to you.

https://www.nature.com/articles/srep44419.pdf 

https://www.nature.com/articles/srep18877

Devapriya

Hi Dinesh,

If you have different types of 96-well plates available you can check and see what type of material the biofilms will attach and grow the best. Some bacteria work better on polyvinyl chloride (PVC) and others on polystyrene. Using the crystal violet method is probably the easiest way to quantify the biofilm inhibition of your nanomaterial.

Also check the supplementation of the media with iron, as in some cases the addition can increase the biofilm formation.

Kind regards