During collagen extraction
EDTA works better at pH~11,0, but you can use em pH7,5 with small efficiency..
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
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01 August 2024 711 1 View
"How has Leader Sheikh Hasina's government allegedly responded to student protests, including the reported killing of over 500 students, as well as arrests, custody, remand, and the involvement of...
29 July 2024 2,181 7 View
My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?
29 July 2024 7,404 1 View
I know the rate law. But for my photo-catalytic experiments, first 90min is adsorption (dark) and next 150min is under UV light (total 240min). How to remove the adsorption part for calculation?...
21 July 2024 8,807 6 View
I want to calculate the spin texture for the electronic band structure obtained from SOC calculation. Is there any way for calculation of spin texture using quantum espresso??
19 July 2024 8,571 1 View
Could you provide information on the average energy consumption per gate operation when using superconducting qubits, specifically focusing on the microwave power required to implement gates? I...
15 July 2024 954 0 View
I've found that some journals are both Scopus-indexed and listed on Beall's list as predatory or potentially predatory. Why does this discrepancy occur? Are there any more reliable platforms than...
12 July 2024 5,158 1 View
All I want to know is whether it is feasible or not. How did genes come to be if they do not come from parents?
12 July 2024 7,221 1 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View
I am intrested in the preparation of collagen carriers, dehydration, paraffin infiltration, and paraffin embedding. Thanks in advance.
05 June 2024 3,831 0 View
I have been making hydrogels regularly using 10X DMEM (phenol red indicator), Ultrapure water, 1N NaOH, and 3.41 mg/ml Collagen (suspended with acetic acid). We have used the same calculations for...
29 May 2024 4,655 0 View
Can rats that were injected with collagenase NPs, show higher collagen deposition than their control untreated counterparts in histopathology examination!!!
11 May 2024 2,931 0 View
I made a myocardial tissue using cardiomyocytes and type I collagen (3 mg/ml). During the culture process, the tissue remodeled and its width decreased. What factors influence the degree of...
10 May 2024 7,784 0 View
hello, I have a scaffold with three layers, two synthetic polymer layers and one central layer made of ECM gel seeded with SMCs. After in vivo culture, how can I determine through Masson's...
05 May 2024 2,297 0 View
how to extract collagen from bones specimen?
27 April 2024 6,307 0 View
Does anyone have guidance on coating 96-well plates with collagen-I? Looking for a protocol. We are using Corning® Collagen I, Rat Tail, 100 mg.
22 April 2024 6,835 1 View
Hi, I have used masson's trichrome staining and trying to analyze collagen staining.. How can we analyze it using Image J. Anyone have the macros available? Thanks
14 April 2024 5,607 2 View