I followed the method described by Yan 2013 (doi:10.3390/molecules18010167):
- 1 ml 0.435 mM brilliant green
- 2ml 0.5 mM FeSO4
- 1.5 ml H2O2 3%
- 1 ml sample
The problem appears when measuring the absorbance of the control (4,5 ml water + 1 ml brilliant green), Abs(control) = 2.3 au, which is too high.
Meanwhile, the Abs (blank) = 1.2 au; the blank was prepared by replacing 1 ml of sample with 1 ml of water.
Thanks in advance!