I followed the method described by Yan 2013 (doi:10.3390/molecules18010167):

- 1 ml 0.435 mM brilliant green

- 2ml 0.5 mM FeSO4

- 1.5 ml H2O2 3%

- 1 ml sample

The problem appears when measuring the absorbance of the control (4,5 ml water + 1 ml brilliant green), Abs(control) = 2.3 au, which is too high.

Meanwhile, the Abs (blank) = 1.2 au; the blank was prepared by replacing 1 ml of sample with 1 ml of water.

Thanks in advance!

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