I have to perform an ultracentrifugation to isolate VLDL and chylomicrons and their remnants from human serum.
Is it possible by just using a KBR-solution with a density of 1.0063 g/cm³? Or do I have to add NaCl/EDTA-solution as well?
How do I know where my top fraction ends? Can I see it?
Do I really have to dialyze it even though I just want to run a quantification?
Thanks a lot!
Thank you very much, but do you know anything about the chylomicrons?
Your protocoll says nothing about the isolation of them. Shouldnt they also appear in the topfraction with VLDL?
https://www.diacomp.org/shared/document.aspx?id=18&docType=Protocol
https://www.cellbiolabs.com/sites/default/files/STA-608-ldl-vldl-and-hdl-purification-kit.pdf
http://www.jlr.org/content/43/2/335.full
I used to separate each fraction .
Chylomicron is white cloudy
VLDL1 &2 r yellow
U can see them clearly
Refers to my phd in ny profile i mentioned the protocol step by step
If u need more details let me know
I recommend that the protocol provided by Dr. Khloud Jamil Ghafouri would be very useful to follow and would help Dr. Paul Schumann vis-à-vis his queries in this regard.
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