How to oxidize/reduce adrenodoxin (or ferredoxin) with small molecules?

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Spermine detection by HPLC?

I tried to detect spermine using reverse phase HPLC and a derivatization agent, but a peak that I want to see on the chromatogramm is very low and is not significantly different from the base...

17 June 2019 5,515 3 View

3D model in Cupcarbon, is it possible?

Dear colleagues, I need to create WSN model in 3 dimensions. I tried to use Cupcarbon for it but I couldn't find this function there. Could you explain me, how to do it in the package? Or to...

08 November 2018 1,605 1 View

What are your opinions about perspectives of oxymoron like pathology diagnosis and treatment?

It’s known, that oxymorons are a proper subsets of the expressions, whose values mutually exclusive, and give us a semantic paradox. We proposed and tested algorithms for diagnosis of oxymoron...

12 October 2018 3,607 0 View

What are your opinions about perspectives of Moebius strip like pathology diagnosis and treatment?

Proposed and tested an algorithm for diagnosis of Moebius strip like pathology, as prerequisite for treatment correction. The algorithm is reduced to initialization of study objects as a...

12 October 2018 5,966 3 View

Where can I physically get a large library of protein genes?

I am studying massively parallel protein-protein interaction. Is there a good source for a ready DNA library, where a protein gene (or part of it) would be surrounded by standard adapters? Like...

05 July 2018 3,018 1 View

fluorescent voltage sensors use?

Dear friends, i have a question regarding the fluorescent voltage sensors. I never tried it before, so I need good advice. What is your experience with Voltage dyes? If would use the...

11 January 2018 5,622 2 View

Do you have a job opportunity in Blockchain area?

Dear colleagues, My current project will finish this December and I'm looking for a new job opportunity in a Blockchain researching area. Write me please if you have an open positions for...

17 December 2017 5,009 3 View

How can I calculate using of energy by WSN node?

Could you write a link where I can find formulas for calculation an energy which is used by node of WSN (for transfer a data)?

17 December 2017 7,814 14 View

What problems are there for implementation blockchain technology in IoT, WSN and mobile devices?

All know that there are a problem POW for small devices. What with what problems have you encountered when implementing blockchain for small devices? What criteria do you use for evaluate new...

12 December 2017 9,748 6 View

We are looking for blockchain researchers.?

Dear colleagues, We are looking for researchers who work with Blockchain technology for taking part in a program committee for Workshop on Blockchain Technologies and Security (BTS) of 9th...

10 December 2017 5,412 31 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View