How can i search for putative homologs of an organism in other genome sequences?

Hello, I need some help. So I want to look for putative homologous structures of some organisms (for example a receptor of a protein) in the complete genome sequence of another organism (a fungus...

30 September 2020 5,368 8 View

Any existing work of PECVD a-Si:H films on crystalline-Si (100) Substrate?

Most all literature discusses a-Si:H films on glass/other substrates. I am interested in any work that may have been done in developing these films on (100) c-Si substrates. I have found one...

04 November 2019 6,034 3 View

First yield criterion in Abaqus?

Dear all, Is there any way to automatically stop a (Riks) analysis in Abaqus when the maximum stress at any point in a model reaches the yield stress? Or when the strain reaches the yield strain?...

25 February 2016 7,695 4 View

How can I stop a Riks step at the maximum LPF in Abaqus?

I have seen this question posted here before, but without a definite answer...I am wondering if it is possible to stop an analysis in Abaqus when the LPF increment is getting negative i.e. if the...

30 September 2015 7,237 7 View

Survey results evaluation (control and two treatment groups)

Hi everyone! I am currently running a survey on socioeconomic topic and among my respondents I have one control and two treatment groups. The treatment is hidden in a survey itself in a form of...

01 January 1970 918 4 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Is nr blast important?

Hello, I have designed a specific primer for my Gene, when I blast this primer, it just finds the mRNA of the gene, but in nr blast, I have found some CDSs that are not relevant to my Gene. Do...

05 February 2021 5,842 3 View

Multiple insertions with Q5 Site-directed mutagenesis?

03 February 2021 221 2 View

How are wild-type and mutant gene and protein isolations and analysis performed?

Considering that we hypothetically designed an experiment, we have an x gene, and we have the mutant version of this gene with a single nucleotide mutation in one of the exons. What kind of an...

22 January 2021 9,589 12 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

How to calculate percent of identity in aligned RNA sequences with a sliding window ?

Hello, I aligned some RNA sequences to identify if some part are similar. I would like to represent my result with the similarity in y-axis and the alignement in x-axis. I want to calculate this...

13 January 2021 7,833 3 View

How to design reverse primer with ambiguous nucleotides?

How to design, reverse primer with ambiguous nucleotides?

03 January 2021 6,762 4 View

Problem executing MrBayes. Help?

Hi, I'm trying to run MrBayes 3.2.7 with a matrix of 37 species and 20561 nucleotides. I consistently receive the following error: MrBayes > Execute...

18 November 2020 3,867 3 View

How can I apply degenerate primer in SerialCloner (2.6.1) to run a PCR?

I have to carry out an in silico PCR on the 16S-rRNA gene of 5 random bacteria strains. I would like to evaluate the resulting amplicon of different primer pairs. Because I can only use free...

14 November 2020 2,341 2 View

What is the mechanism of RNA fragmentation?

https://www.thermofisher.com/order/catalog/product/AM8740?SID=srch-hj-AM8740#/AM8740?SID=srch-hj-AM8740 RNA Fragmentation Reagents The average size of the resulting fragments will be 60-200...

12 November 2020 4,195 2 View