I have collagenase from sigma product code c9891 100mg,Type IA, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid, For general use
I do not understant from the product data sheet how many units are per mg (U/mg)....
How can I make a 30U/mL solution? How much buffer should I add to what quantity of powder to obtain 100mL at 30U/mL solution?
I would appreciate your experience. Thank you!
Go the the Sigma web site for the product you received and enter the lot number in the certificate of analysis (COA) window. The COA may give you specific numbers for the particular lot number in your possession.
Then, you will have to decide which type of unit applies to your experiment, and you can use that to make the calculation.
For example, suppose the material has a specific activity of 10 units/mg solid and you want 100 mL at 30 U/mL. The calculation goes like this:
(100 mL x 30 U/mL)/(10 U/mg) = 300 mg.
Gently dissolve 300 mg in sufficient buffer to bring the volume to 100 mL. Avoid as much as possible making foam when dissolving the protein.
- Badges
- Science topic