How to optimise soft agar assay?

17 June 2019 0 5K Report

I would like to test if immortalised cells can transformed to cancer cells after certain drug treatment. I looked protocols online and each protocol suggests different % agar etc. I just used the same DMEM 1X with FBS to culture my cells to make soft agar and only have seen one colony and I would like to optimise the protocol. Based on what would you change % agar or % FBS? BTW, I use RPE-1 cell for my experiment.

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