Anna Middleton

3 Questions 1 Answers 0 Followers

Questions related from Anna Middleton

How can you select the combinations of allogeneic cells for Two-way MLR?

I have PBMCs from different donors but no serum. Can I still use lymphocyte crossmatching by flow cytometry method?

27 January 2022 893 0 View

Can anyone share good Two-way MLR Assay protocol?

Hi, I am trying to set up two-way MLR assay. Simply mixing two PBMCs which are stained with proliferation dye and leave it for 5 days and run it on FACS. Does anyone can share good protocol for...

16 November 2021 9,887 1 View

How to optimise soft agar assay?

I would like to test if immortalised cells can transformed to cancer cells after certain drug treatment. I looked protocols online and each protocol suggests different % agar etc. I just used the...

17 June 2019 4,930 0 View