I am encountering some challenges in the process of conducting a primary selection that can effectively distinguish the target DNA bands between pest -resistant and pest-susceptible varieties against the top borer. Despite my efforts, which involved using 1 primer and 3% agarose gel, as w ell as 1 primer with 3 different restriction enzymes, I have not ye t achieved a specific target band pattern on the 3% agarose gel. I would greatly appreciate any information or guidance regarding the issue I am facing. Additionally, I am seeking information on the resistance criteria of sugarcane varieties based on field observations. The resin stant sample also includes the use of wild sugarcane (S. spontaneum) in the first lane of the gel. Your kind assistance would be invaluable in helping me address these challenges. Thank you in advance for your consideration and support.
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