I am normalizing with respect to the G band, in this way I obtain as Raman intensity for this band a value of 1.00 and for the D band a value of 1.42, in this way I calculate ID / IG = 1.42, I don't know if this procedure is correct .
yes, it is correct but I don't think you need to normalized data first. You should just draw graph and note ID and IG
Go to the origin, Calculate the area of D band and G band (Can be done by curve fitting) Divide both to get Id/Ig ratio.
D band intensity (ID) is equal to the area under the D band curve?
I am looking for expert recommendations on qualitative analysis software. I have been engaging in more mixed-method work recently and I need reliable qualitative analysis software. Any...
02 February 2021 7,984 7 View
Hi there, I am looking for some advice with FISH. This is my first time attempting this technique and I am not too sure where I could potentially be going astray. I have followed FISHTag RNA...
23 September 2020 3,849 1 View
We are looking to do a study on leadership and we want to use a an Emotional Intelligence Scale that meets the following criteria 1. is used in published academic research, not just consulting...
14 July 2020 7,122 2 View
I am working with N-Benzyl substituted indazole-nucleus but I'm having many problem with the stability of the molecules. Does anyone know if the benzyl position is sensitive to something, such as...
29 June 2020 7,505 3 View
I'm having a heck of time! I'm trying to perform triple immunofluorescence for CAMKII (alpha; mouse; milipore 05-532; 1:5K; visualized with AlexaFluor 647), Fluorescein (Invitrogen, A11095, goat,...
29 October 2019 7,063 2 View
The size of protein X (Phosphorylated and non-phosphorylated) will be pretty much the same on a western. Let's say after primary antibodies incubation of the two separate proteins (Phosphorylated...
16 September 2019 5,583 5 View
Hi, I am having trouble with my Oil Red O stain for my rat MSCs. What is the best aqueous mounting medium to use? I followed the protocol from IHCWorld...
03 April 2019 10,012 3 View
Hello all, I am completing a paper on the change in impairment over the course of treatment. I am not only interested if seeing that there is change overall, but where the biggest change occurs...
24 February 2019 3,709 4 View
Hi, I am planning on seeding rat MSCs onto 6 well plates with coverslips attached on the bottom. I then want to use stains such as Alizarin Red and Von Kossa to see the level of osteogenic...
02 February 2019 7,584 1 View
I am totally confused after beginning this course a week ago. However, I want to fully understand so that I can make the best grade possible. I have considered topics dealing with Bullying,...
13 January 2019 5,876 5 View
In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...
01 March 2021 2,207 3 View
Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...
26 February 2021 4,963 5 View
During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...
25 February 2021 7,994 3 View
I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...
24 February 2021 363 3 View
How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...
24 February 2021 166 6 View
Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...
24 February 2021 3,785 3 View
I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...
23 February 2021 4,155 4 View
My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...
22 February 2021 2,965 4 View
Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...
22 February 2021 8,132 6 View
I want to illuminate a scene (small space) using LED to capture photographs or videos of some tiny objects inside the space. But am not sure if I should use a Laser LED, or simple LED or power...
22 February 2021 4,240 4 View