Hello!

I'm looking for a good way to mount mouse cochlea(PFA fixed and immunofluorescence stained already).

I examined mouse cochlea immunohistochemical, divided cochlea into some pieces.

Cochlea has a strong spiral shape, also has springy part and weak part, these things make hard to mount with coverslip.

It creates cochlea cells(on weak part) tilt when covered with a coverslip, it's difficult to observe the clear shape of the cells(I mean hair cells).

Does anybody have tips to mount thick samples like cochlea ?

Thanks in advance!