Hi, I'm trying to take picture after immunolocalization with insect guts. After I did all process, I have a problem when I mount the guts. I used pipette and pipette tip (I cut the tip) to do this. But, most of the guts stuck to the tips.. Do you have any idea to solve this problem?
Hi June-Pyo,
I would try to fix the tissue (whole-mount) after dissection in 4% PFA for 20-30 mins, then wash with PBS 3 times, and transfer the tissues with glass or metal hooks.
best wishes,
Refik
Refik Kanjhan Thank you for sharing your experience. What are the exact name of glass or metal hooks? I cannot find them on google.
June-Pyo, I don't know any specific names or suppliers, but you can get some from people who works with glass or metal. For example, glass blowers know how to make simple tools like that in a few minutes by melting and pulling the glass. You may also be able to make one yourself by heating and pulling a tall piece of glass (eg, pipette), though you should be careful as it is potentially dangerous. It is better to find someone who knows what they are doing.
Refik
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