My work is on biomolecule sensing by potentiometry (CA, CV, etc.). Concentration of each individual biomolecule can be easily monitored by variation in current or voltage, but how to check selectivity i.e. which specific biomolecule is present in a mixture of different biomolecules. In case of heavy metals the redox potential difference is high which is not the case in biomolecules. So please suggest me how to sense or discriminate specific biomolecule (ex. amino acid).
Hi Ishu Singhal ,
I have few suggestions for you.
You must do (know) the following:
1. What is the charge of your molecule (analyte) at the working pH? (you can get this from the pka of the molecule).
2. What are the charges of the other interfering molecules.(you can get this from the pka of the molecules).
3. Does your molecule have specific interactions such as pi-pi with your electrode material or thin film.
4. Are there any materials in the literature that showing selectivity towards your analyte.
5. Design an electrode material based the factors above.
6. Use Differential pulse voltammetry technique to know the selectivity of the platform towards your molecule.
7. Do some control experiments such as blank, molecule of interest, and the interfering molecule one by one. Compare their responses and come to a conclusion.
I hope that these steps will surely help you.
Have a great time...
Thank you very much for your prompt suggestions Dr. Vusa. I will follow these steps and will share the findings with you for sure.
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