How does trust influence the development of environmental projects?

Hello Scholars, I am exploring "trust" relevance in the development of socio-technical systems. What is your perception of cognitive values like trust to the energy transition/implementation of...

09 February 2021 8,133 5 View

How to increase AZ 40XT adhesion on conductive metal?

05 October 2020 2,899 3 View

What are the nuclei mean number from freeze tissue nuclei isolation protocol?

Hello, everyone! I'm going to start a project with single-cell ATAC-seq for analysis of open chromatin regions from freeze tissue samples. I'm lost about the nuclei concentration. If I made a...

19 June 2020 7,348 2 View

Can I have a list of genes from differents genomic coordinates?

Hello, I have a list of genomic coordinates from differentially methylation regions and a I need to extract from them a list of genes, and I don't want do look one region by one. Is there a R...

02 January 2020 7,058 4 View

What is the best method for mammalian blood RNA extraction?

Hi everyone, Does anyone have protocol for mammalian blood RNA extraction with Trizol? I have not obtained good results. Thanks

02 September 2019 2,722 3 View

Problems on primers validation?

Hello, I'm validating the primers for GAPDH, Tubulin and HSP70 with 8-fold serial dilution (1: 2) and the efficiency is very high and with points outside the standard curve. I read about reagent...

16 March 2018 5,176 5 View

What program can I use to make a in silico PCR and throw me as result an agarose gel with my expected fragment?

What program can I use to make a in silico PCR and throw me as result an agarose gel with my expected fragment?. Only putting my sequence of interest, my primers and the expected size.

12 September 2016 3,379 1 View

Can someone better explain to me how the reaction of amine halide and the surface of graphene oxide works?

I have been using trimethylamine to functionalize graphene oxide membrane and PPO, but I can't see the reaction of amine halide on the graphene oxide surface.

01 January 1970 478 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View