Hi,
I am creating BSA microparticles in organic solvents using a process known as microglassification. These microparticles are practically pure protein with no additives, stabilisers, surfactants etc.
When I attempt to dry and collect these particles I observe a large amount of irreversible particle aggregation. Mechanical operations such as pelletisation via centrifugation also result in large-scale aggregation.
My most successful technique to date is to centrifuge at low speed for 30 mins to separate (but not pelletise) , remove as much solvent as possible and leave the sample to dry by evaporation. However, the evaporation takes around 1 week to complete as I am using pentanol as the organic phase.
Can anyone suggest other techniques to dry and collect my particles or techniques to reduce the propensity to aggregate (note: these particles cannot be suspended in aqueous media as they are water-soluble).
Thank you
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