Thomas S Booth

6 Questions 3 Answers 0 Followers

Questions related from Thomas S Booth

Are these two preparations of phosphate buffer the same?

I am attempting to replicate a HPLC method for the determination of water-soluble vitamins and the mobile phase consists of 0.1M Phosphate buffer (pH 7). The paper states that they made up the...

20 October 2022 1,809 2 View

What methods can be used to measure the phase transition temperature of natural lecithins?

I would like to measure the phase transition temperature of sunflower lecithin from the gel phase to the liquid-crystal phase. The supplier of the product has advised that it is expected to be...

08 April 2022 7,019 3 View

Can I dissolve freeze-dried catalase and lysozyme in de-ionised water or do I need a buffer?

Can I dissolve freeze-dried catalase or lysozyme directly into de-ionised water or do I need to use a buffer (separate solutions, not both proteins in one)? I will not be storing the solutions and...

11 November 2020 3,349 5 View

How do I report an average standard deviation over multiple samples?

I have a triplicate data set in which each set of data has a mean and standard deviation. I want to report the mean of the means but the error should reflect the standard deviations within the...

27 June 2020 2,690 0 View

Can I remove water from 1-pentanol using RotaVap?

Hi, I am using pentanol as the solvent phase in a liquid-liquid system to create microparticles by dehydration of aqueous droplets. I would like to find a way to reuse the pentanol, which means...

19 February 2020 4,646 0 View

How to minimise protein microparticle aggregation?

Hi, I am creating BSA microparticles in organic solvents using a process known as microglassification. These microparticles are practically pure protein with no additives, stabilisers, surfactants...

09 December 2019 7,246 0 View