which is the best ethanol assay kit for plasma/ serum?
Hi everyone, I’m trying to design a plasmid that, in combination with a viral vector, might prevent the involution of the thymus in humans. I’m going off this paper where they gave some mice a...
31 July 2023 2,513 1 View
I am looking to make RMT graphs using R based on Raubenheimer (2011). https://esajournals.onlinelibrary.wiley.com/doi/abs/10.1890/10-1707.1 I have used the PrimateEats/balancr package in R. Does...
23 December 2022 2,408 4 View
I used clustal emboss transeq to convert 3 viral DNAs into amino acid. I checked 6 frames in the choices, and I got got 6 results. Should I align all 6 frames of the 3 viruses? Or should I select...
19 February 2020 275 2 View
I have a geospatial PDF map & it opens very slowly in Acrobat 2017. (60 seconds or so) What are my best alternatives? The map is very powerful allowing me to search on road numbers, lat longs,...
09 July 2019 5,177 5 View
I'm looking for company that can professionally segment the neurovasculature from some CT angiograph DICOM data. Thank you in advance!
02 July 2019 3,750 3 View
I am looking for a good Elisa Kit for assessment of IL-6 in plasma and brain samples
13 December 2018 5,004 0 View
Hi Doris, my name is Callum Fletcher and you may remember that couple of years ago, I spoke with you about developing a suction trap network around Australia. We received funding from the Federal...
23 October 2018 9,588 1 View
I am performing an elisa insulin assay (demeditec). the washing procedure appears to be crucial in this kit .
11 December 2017 3,802 1 View
PCR gel electrophoresis PCR trouble-shooting
16 April 2016 1,043 6 View
I have designed a variant of a Genetic Algorithm, what are the standard functions I should use to test it on?
30 March 2016 7,226 5 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
