Add 10 µl of spore solution to each side of the hemacytometer.
Each large squares in the chamber has space to accommodate 0.1µl of suspension. Using unitary method we can thus calculate average number of spores per ml of the suspension.
Determining spore count:
Count the number of spores on each large squares at the corners.
Calculate total number of spores.
Calculate spore count as follows:
spores per ml of suspension = average spore count per large square x 10∧4.