Amit Kumar For calculating AS for PCF based SPR sensor, we use SA = -(1/alpha( λ , na )* ( del alpha ( λ , na )/ del na) (RIU^-1), where alpha( λ , na ) signifies the confinement loss at a particular refractive index (RI) of the analyte, del alpha ( λ , na ) is the the difference between two adjacent loss spectra due to a small change in the refractive index of the analyte, del na is the change of the refractive index of the analyte . You can check the following paper as a reference. Thank you
SPR introduces a dramatic shift in relative phase difference between the s- and p-polarization components at a specific wavelength. Meanwhile, the phase of the s-polarization component remains unaffected by SPR. The interference spectrum detected is determined by the OPD between the p- and s-polarized components. According to Fresnel formula [17,18], the interference spectrum can be expressed as: I(λ) = I0(λ){1 + VSPR(λ) cos[φOPD + φSPR(λ)]} + INoise (1) in which I0(λ) is the light source spectrum, VSPR(λ) is the interference fringe contrast, φOPD is the additional phase difference between the p- and s-polarization components introduced by the birefringence crystal, and φSPR(λ) = |φp − φs | is the SPR phase.