How to measure PLA-2 and PAf-AH activity in plasma samples of diabetic patients?

21 August 2014 1 3K Report

Hi all,can anyone please suggest me a suitable method to measure specific PLA-2 and PAF-AH activity in plasma samples of diabetic patients.

Juan Gómez Gerique

Please See: https://www.researchgate.net/publication/6422383_Platelet-activating_factor_acetylhydrolase_%28PAF-AH%29_activity_in_patients_with_type_1_diabetes_mellitus. I Think would be of interest for you.

Article Platelet-activating factor acetylhydrolase (PAF-AH) activity...

Badges
Science topic

More Alpesh Thakker's questions See All

How many percentage of ammonium sulphate saturation can be achieved in partial purification of cellulase while using CMC broth for enzyme production?

I'm facing difficulties in the partial purification of cellulase enzymes. I used 2% CMC broth as a production medium, and after the separation of the pallet crud enzyme was saturated with ammonium...

22 January 2023 1,584 0 View

Appropriate solvents to be used in HPLC/GC-MS?

My team and I are new to HPLC/GC-MS quantification so we would appreciate some insight. Currently, we are trying to measure the concentration of organic acids within an organic solvent...

07 November 2021 1,198 3 View

I use LB agar supplemented with KL and methylene blue as indicator dye to screen lignolytic bacteria. can i add methylene blue before autoclave?

for microbiological media preparation, methylene blue is added before or after autoclave????

24 July 2020 8,916 1 View

For supervised classification using Object based method, How many images of same scene are required ? Can I do OBIA using one Image?

In object based classification method , first Image is segmented in no of objects and then this objects are used as training , validation and testing data. Do I need data sets of Images of Same...

25 October 2018 5,920 3 View

How to investigate the efficiency and stability of tbdms derivatisation in GCMS analysis technique?

Amino acid analysis and/or organic acid analysis using GCMS method post derivatisation with MOX reagent and MTBSTFA reagent is routinely used by several labs. However, I was not able to find a...

05 June 2016 4,143 2 View

How can I normalise mass spectrometry data (direct infusion and LC-MS)?

I performed a direct infusion and LC-MS analysis of oxidised phospholipids mainly PC and PE class using various reverse phased columns for biological samples and in-vitro samples. I do not add any...

02 April 2014 566 8 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View