Leptin is one of the main satiety factor in mammalian systems, its concentration is positively correlated with white adipose tissue mass (ie: BMI).
I'm studying Leptin in the context of CVD. Serum samples are drawn from patients and Leptin concentration is measured using ELISA.
What is the equation or calculation used to normalize my reading with respect to BMI?
Dear Faek Ali,
Maybe the following papers will help you on the subject:
Ruhl CE, Everhart JE, Ding J, Goodpaster BH, Kanaya AM, Simonsick EM, Tylavsky FA, Harris TB; Health, Aging, and Body Composition Study. Serum leptin concentrations and body adipose measures in older black and white adults. Am J Clin Nutr 2004;80(3):576-83. https://academic.oup.com/ajcn/article/80/3/576/4690533
Ruhl CE, Harris TB, Ding J, Goodpaster BH, Kanaya AM, Kritchevsky SB, Simonsick EM, Tylavsky FA, Everhart JE. Body mass index and serum leptin concentration independently estimate percentage body fat in older adults. Am J Clin Nutr 2007;85(4):1121-6. https://academic.oup.com/ajcn/article/85/4/1121/4648768
Nazish Rafique, Mohammad Nasir Afzal. Relationship of serum leptin levels with body mass index and gender. Rawal Medical Journal 2009;34(2):1-11. https://www.ejmanager.com/mnstemps/27/27-1302359611.pdf?t=1552645414
Shah NR, Braverman ER. Measuring adiposity in patients: the utility of body mass index (BMI), percent body fat, and leptin. PLoS One 2012;7(4):e33308. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317663/pdf/pone.0033308.pdf
Cohen SS, Fowke JH, Cai Q, Buchowski MS, Signorello LB, Hargreaves MK, Zheng W, Blot WJ, Matthews CE. Differences in the association between serum leptin levels and body mass index in black and white women: a report from the Southern Community Cohort Study. Ann Nutr Metab 2012;60(2):90-7. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710998/pdf/anm-0060-0090.pdf
Khan Tabassum Tanvir, Mohd Muzaffer Ali Khan , Mohd Inayatulla Khan. Correlation of Serum Leptin levels with Body Mass Index and Gender. J Cont Med A Dent 2016;4(1):54-57. http://www.jcmad.com/allpapers/419a12.pdf
“Leptin values are appromiately 2.5 times higher in women than men per unit BMI.” http://www.kumc.edu/Documents/siddrc/dma/immuno_PDF/leptin%20human.pdf
Best wishes from Germany,
Martin
Hello, I'm a medical laboratory specialist in the field of clinical chemistry. I'm also going to measure serum leptin for patients with breast cancer; Could you kindly help me where to find the ELISA kit? And if it is possible please send the principle of the kit.
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