I've seen some approaches such as using luciferase assay and ChIP-seq data, but I'm not sure which one to use.
If you are interested in the activity of a few well defined enhancer regions then wet lab assays are the way to go. However, if you are interested in a broad range of regions then ChIP-Seq would be a better choice between the two approaches.
ChIP-seq is the way to go. Targeting H3K27ac and H3K4me1 will give you location and activity of enhancers. It can be combined with ChIP-seq on CTCF and ATAC-seq for open chromatin map if you can afford.
Diagenode has ChIP-seq grade antibodies for ChIP-seq and also offers services.
https://www.diagenode.com/en/categories/chip-seq-service
Thank you both!
Geoffrey Berguet, I'm not familiar with ChIP-seq, ATAC-seq and capture-C data. Do you have any tips on how to learn, any article, book chapter, tutorial, etc you'd recommend?
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