How to determine the cell density or total suspended solids of the bacteria which are entrapped in hydrogel like PVA+Alginate?
Hi,
I hope that these articles will be useful. Best Luck.
Article Isolated Reporter Bacteria in Supramolecular Hydrogel Microwell Arrays
Article Metabolic Responses of Bacterial Cells to Immobilization
You can read those articles online.
Article Rheology of peptide- and protein-based physical hydrogels: A...
https://www.ncbi.nlm.nih.gov/pubmed/25266637?dopt=Abstract
Article Control of Three-Dimensional Cell Adhesion by the Chirality ...
Article Micropatterning of Hydrogels by Soft Embossing
Thank you Badreddine, Midhtak and Traci for the suggestions! Will try out these methods and see which one work the best for my experiment.
I am trying to use CRISPR and the Lambda red system for gene knockout in Klebsiella. I would like to seek advice on how to design the repair template. If anyone can advise me on this I highly...
05 May 2024 344 0 View
If anyone can advise me on any protocols which work best for Klebsiella gene knockout are welcome.
05 May 2024 2,800 0 View
I am new to this concept and I need some guidance on designing the primers with restriction site, homologous region, and antibiotic resistance gene casseettee. I am also looking for the suicide...
03 March 2024 8,193 2 View
Thanks
28 February 2024 6,562 3 View
Request for scale on political economical and social factors
13 February 2024 2,755 1 View
Any other best therapeutic strategy for multiple myeloma
03 January 2024 4,628 3 View
I'm confused about 'homogeneity of cellular transduction' of AAV. Does this mean AAV can only transduce one certain type of cells after it enters into living organism?
15 May 2023 8,675 3 View
I am searching for relative information about Nestle's contribution on climate and the environment. I wonder if the company has ever done some negative or opposite to the goal of protecting the...
14 April 2023 5,901 1 View
Can anyone in the research gate community share contact details of personnel who works in NIBSC.org please. I need some details regarding there "Genetic Reference Panel, for the quantitation of...
04 November 2022 9,177 0 View
Sanger Sequence trace
29 July 2022 7,725 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
What is the behavior of anaerobic bacteria in deep salty groundwater with high level of chlorine? Is the nitrate level deceasing and is denitrification controlled by excess chlorine? Thanks
13 July 2024 1,581 4 View