Why oxidation with peracetic acid engender staining of oxytalan fibers with clastic tissue stains?

Just a quick question above, this oxidation method is mentioned by literature, but no one talked about the principle. Can anyone figure out how the oxidation works on any specific group?

20 December 2020 5,878 1 View

Why does my HPLC filter always generate air bubbles?

I have an old HPLC machine. Every time when I put my filter into 50%:50% water and acetonitrile mixture, my filter always generate bubbles. However, once I change the solvent to 100% acetonitrile,...

08 March 2020 5,587 4 View

What are those clay/silt aggregates? Are those intraclastic clast or flocs?

These deposits were from mud caps of sediment gravity flow deposits. These mud caps are homogenous and no trace fossils can be identified. Can any one help me identify these aggregates? Thank you...

10 September 2018 3,627 3 View

How to compute the band structure of TM mode for 2D photonic crystals by COMSOL?

Can we use the COMSOL to compute the band structure of TM polarization for the 2D photonic crystals? If yes, how to do it? With the "Bandgap Analysis of a Photonic Crystal" in application gallery,...

19 September 2017 1,620 1 View

Why do the same primer real time PCR amplification efficiency is not the same every time?

Why do the same primer real time PCR amplification efficiency is not the same every time?

18 September 2017 8,644 2 View

Flame Retardant materials

I am looking to find commercial available Flame retardant materials so that I can implement them in my plastic pallets which are HDPE-silica composite. The presence of silica do enhance the FR...

09 July 2017 2,900 1 View

"Inflation and the Measurement Problem" written by S. Alexander, D. Jyoti, J. Magueijo. Why their model solve the cosmological measurement problem?

02 May 2017 9,389 3 View

Pathloss model for mobile femtocells?

23 November 2016 9,904 3 View

How to calculate Power of Rayleigh Channel / Rayleigh distributed signal (multipath effect)?

27 October 2016 3,668 5 View

Do intestinal flora affect the DNA or RNA detection of colorectal cancer? why?

Intestinal flora will affect the DNA or RNA detection of colorectal cancer?

28 August 2016 2,998 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View